Journal: Neuroscience Insights

Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments

doi: 10.1177/26331055241288172

Figure Lengend Snippet: Arteriolar deposition of fibrillar amyloid and loss of smooth muscle cells in rTg-D rats. Brain sections from a WT rat (20 M) (A, C and E) or similarly aged rTg-D rat (22 M) (B, D and F) were immunolabeled with antibodies to collagen IV to identify cerebral blood vessels (red) or to smooth muscle cell α actin to identify smooth muscle cells (green) and stained with AmyGlo to visualize fibrillar amyloid (blue). Representative images from pial arteries (A and B); cortical arteries (C and D); and cortical arterioles (E and F). Scale bars = 50 µm.

Article Snippet: Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems).

Techniques: Immunolabeling, Staining